Core Facility Equipment

The Eppendorf epMotion 5075 liquid handler system is used to aliquot DNA samples, chemical reagents and buffers to automate molecular biology protocols. Both 96-well Matrix and skirted plates can be used for downstream analysis on other instruments, such as next-generation sequencers, PCR machines or SNP analysis.

The epMotion 5075 improves the efficiency and safe handling of DNA samples by accurately pipetting samples in sterile conditions and aliquots in a fraction of the time required for a technician to manually aliquot a sample order. The bonus feature of the system is that it can pipette up to four plates.

Eppendorf epMotion 5075 Liquid Handler

• Provides walk-away automation for multiplate replication pipetting
• Replicates 96-well microplates and tubes
• Capable of replicating one 96-well microplate in less than 1 minute
• Automatic multipipette tool exchange
• Workbench has up to six plate positions

The Hamilton Verso Q20 freezer system can store up to 22,000 at -20C for long-term archival purposes. Samples can be quickly retrieved when needed, and the buffer location allows for temporary holding when plates are pulled. In conjunction with the epMotion 5075, it is easy to aliquot your sample collection into 0.75mL Matrix tubes, then store them in the Verso Q20.

The Hamilton Verso Q20 freezers feature the following:

• Fully automated sample storage
• Stores 0.75ml Matrix tubes at -20C
• Retrieves samples by robotic cherry picking
• Retrieval time is up to 390 tubes/hour
• Utilizes INSTINCT S Software in conjunction with NUgene’s sample database
• Utilizes an internal plate reader for sample introductions to update inventory
• Sample Storage in -80C Freezer Farm

NUgene also offers sample storage for any sample type at ultra-low temperatures. Our Thermo Fisher Forma 903 upright freezer can store DNA, blood, serum, chemicals and tissue samples at -80C. Our -80C freezer is in the freezer farm of the McGaw basement.

The Perception HD plate reader assists in identifying retrieved or newly aliquoted DNA sample DataMatrix barcodes of 0.75mL DNA Matrix tubes

• Scans DataMatrix barcoded DNA storage tubes (0.75mL) in seconds
• Connects to PC for exporting .csv files noting DataMatrix barcode and well locations
• Easily integrates with NUgene’s new Hamilton Verso Q-Series