ChIP-seq is a genomics technology developed to map binding sites of a DNA-interacting protein across the genome. Examples of DNA-interacting proteins include transcription factors, histones, and enzymes for DNA repair and modification. A common application of ChIP-seq is to locate transcription factor binding patterns under different conditions, such as development stages or pathological conditions.
ChIP-seq starts with covalent cross-linking of DNA with interacting proteins, then shearing of chromatin into fragments, followed by enrichment of the protein of interest with its bound DNA by immunoprecipitation using an antibody specific for the protein. Subsequently, after dissociating the enriched protein-DNA complex, the released DNA fragments are subjected to sequencing. One key experimental factor in the ChIP-Seq process is the quality of the antibody used in the enrichment step, as the use of a poor-quality antibody can lead to high experimental noise due to non-specific precipitation of DNA fragments.
NUSeq accepts ChIP DNA, as well as input DNA, for sequencing library construction and then sequencing. Please see below for sample submission guidelines.
ChIP-seq Library Prep Pricing
Library prep costs are listed on the Core Pricing page.