Northwestern University Feinberg School of Medicine

Center for Genetic Medicine

Events

Nov

08

ChBE Seminar Series: Laura Segatori

Evanston - 9:00 AM - 10:00 AM

Laura Segatori of Rice University.

Host: Danielle Tullman-Ercek

Title: 
Engineering synthetic regulatory systems to reprogram mammalian cells

Abstract:
The chemical and energetic properties specified by a protein’s amino acid sequence and encoded by our genome, while determining the protein folding energy landscape, are only part of what shapes how proteins evolve their function. A sophisticated network of macromolecular assistants is needed to control protein synthesis and folding, and mediate degradation of aberrant proteins – functions achieved through completely different and independent mechanisms balanced extrinsically by chemical feedback agents. We seek to develop novel biotechnologies to manipulate these mechanisms for applications ranging from systems-level investigations of protein function to development of therapeutic approaches for restoring cellular homeostasis. We employ synthetic biology tools to develop genetic circuits that interface with pathways that mediate protein folding and degradation (e.g., the Ubiquitin-Proteasome System and the Unfolded Protein Response) with the ultimate goal to monitor and manipulate cellular protein levels with high specificity, sensitivity, and dynamic resolution. We recently developed a technology for degrading a target protein with high specificity and selectivity that provides dynamic control over protein accumulation and that can be customized to target any cellular protein and post-translational modifications (ACS Synth Biol. 2018 Feb 16;7(2):540-552). We have deployed this technology to improve the design of synthetic gene circuits through stimulus-dependent, targeted depletion of the circuit reporter and, ultimately, develop a universal platform for monitoring changes in gene expression with high sensitivity and dynamic resolution. I will discuss the use these tunable cell-based platforms not only to monitor and engineer cellular protein degradation but also to generate novel tool for the mammalian synthetic biology community.

Biography:
Laura Segatori is an Assistant Professor of Bioengineering at Rice University with joint appointments in the departments of Chemical & Biomolecular Engineering and Biosciences. She received a Laurea in Industrial Biotechnology from the University of Bologna in Italy in 2000 and a PhD in Chemical Engineering from the University of Texas at Austin in 2005. She completed her postdoctoral work at The Scripps Research Institute in La Jolla California and joined the faculty at Rice University in 2007. She is the recipient of the NSF CAREER Award. Her research group is highly interdisciplinary and combines principles and tools from engineering and science to decipher and manipulate cellular quality control mechanisms that underlie the development of human diseases. Current research interests include the design of synthetic biology tools and nanotechnology-based approaches to engineer protein degradation pathways in the complex environment of mammalian cells.

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Nov

09

Simpson Querrey Center for Epigenetics Forum on Biochemistry, Epigenetics, and Metabolism (BEaM)

Chicago - 3:30 PM - 5:00 PM

The BEAM Forum is a data group where Simpson Querrey Epigenetics Center members present ongoing work being conducted in the Center. It is a great opportunity to get feedback on your work from colleagues, and to make new connections and foster new collaborations within the Center.

The forum is normally held every other Friday from 3:30-5:00 pm throughout the year in Baldwin Auditorium. Two presenters will give ~30 minute talks each week with 10 minutes for discussion and questions. We invite all labs associated with the SQE Center to participate. We welcome presentation from students, techs, postdocs, and PIs. Presenting work-in-progress is always encouraged!

Pizza and soda is provided.

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Nov

12

Pathology Monday Noon Research Conference - "The Role of Chromatin Boundary and HOX-associated lncRNA in HSC Function and Leukemogenesis"

Chicago - 12:00 PM - 1:00 PM

303 E. Chicago
Ward Building - Conference Room 3-015

Guest Speaker:
Suming Huang, PhD
Professor of Pediatrics and Pharmacology
Four Diamonds Endowed Chair, Epigenetics and Gene Regulation Research Program
  

"The Role of Chromatin Boundary and HOX-associated lncRNA in HSC Function and Leukemogenesis"


Light lunch will be provided.

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Nov

12

Pulmonary & Critical Care Medicine Presents - Research-in-Progress Fall 2018 Series

Chicago - 12:00 PM - 1:00 PM

The Division of Pulmonary & Critical Care Medicine at the Feinberg School of Medicine Presents:

Research-in-Progress

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Nov

15

From GWAS to Gene: Adventures in C.elegans Quantitative and Evolutionary Genetics

Chicago - 1:00 PM - 2:00 PM

Erik Andersen, MD

Assistant Professor, Department of Molecular Biosciences

Northwestern University

Evanston Campus

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Nov

19

“Using Big Data to Detect Clinical Deterioration” - Matthew M Churpek, MD, MPH, PhD - Pulmonary & Critical Care Medicine Presents - Research-in-Progress Fall 2018 Series

Chicago - 12:00 PM - 1:00 PM

The Division of Pulmonary & Critical Care Medicine at the Feinberg School of Medicine Presents:

Research-in-Progress

“Using Big Data to Detect Clinical Deterioration”
Matthew M Churpek, MD, MPH, PhD
Assistant Professor of Medicine
Pulmonary & Critical Care Medicine Section
University of Chicago

Monday, November 19, 2018 - Noon to 1:00 PM
Canning Auditorium, 3rd Floor
Prentice Women’s Hospital
250 E Superior St
Chicago, IL 60611

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Nov

20

BMG Faculty Candidate, Dr. Evgeny Kvon

Chicago - 10:00 AM - 11:00 AM

Evgeny Kvon, PhD
Project Scientist, Environmental Genomics and Systems Biology Division
Lawrence Berkeley National Laboratory, Berkeley, CA

Title: Distant-Acting Enhancers in Evolution and Disease

Abstract:

Transcriptional enhancers are a predominant class of non-coding functional DNA elements that are critical for controlling when and where genes are expressed. Changes in enhancers have been associated with morphological evolution in vertebrates, but specific molecular events associated with major vertebrate morphological transitions have remained elusive. We utilized a series of recently sequenced vertebrate genomes and genome editing to study the molecular and functional evolution of a critical limb enhancer and its role in limb loss in snake lineage. A specific subset of nucleotide changes within the enhancer contributed to its functional degeneration in snakes demonstrating changes in a regulatory sequence associated with a major body plan transition. This work provides a powerful experimental approach to study the role of enhancers in vertebrate evolution.

Changes in enhancer sequences have also been implicated in human disease. However, due to the lack of high-throughput in vivo assays, establishing causal links between human phenotypes and enhancer variants identified from human genetics studies remains a significant challenge. We used large-scale enhancer-reporter assay to functionally assess in vivo nearly a thousand human enhancer variants reaching close to saturation of possible mutagenic events. We show that 65% of all candidate rare non-coding variants previously implicated in polydactyly drive reporter gene activity in a pattern consistent with their pathogenic role. We also show that many base pairs spread across the entire length of the enhancer are critical for its function and uncover numerous novel gain-of-function mutations that have not yet been observed in patients. These results illustrate the power of large-scale transgenesis to comprehensively interrogate how non-coding variants affect human biology.

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Nov

26

Pulmonary & Critical Care Medicine Presents - Research-in-Progress Fall 2018 Series

Chicago - 12:00 PM - 1:00 PM

The Division of Pulmonary & Critical Care Medicine at the Feinberg School of Medicine Presents:

Research-in-Progress

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Nov

27

Microbiology-Immunology Department: Nels Elde, PhD

Chicago - 12:00 PM - 1:00 PM

Title: "The Evolutionary Potential of Poxviruses"

Nels Elde, PhD - University of Utah

Host: Virology Graduate Students/ Coordinator Nathan Meade, PhD (Lab of Derek Walsh)

Description:

We use experimental evolution, genomic analysis, and related approaches to investigate how poxviruses adapt to counteract host immune defenses. The work is revealing an array of recombination-driven processes and mechanisms of adaptation involving horizontal gene transfer. Our studies highlight the unique biology of poxvirus evolution.

 

 

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Dec

03

“Methods for spatial transcriptomics analysis” - Valentine Svensson, PhD - Pulmonary & Critical Care Medicine Presents - Research-in-Progress Fall 2018 Series

Chicago - 12:00 PM - 1:00 PM

The Division of Pulmonary & Critical Care Medicine at the Feinberg School of Medicine Presents:

Research-in-Progress

“Methods for spatial transcriptomic analysis”
Valentine Svensson, PhD
Postdoctoral Scholar
Biology & Biological Engineering Division
Caltech

Monday, December 3, 2018 - Noon to 1:00 PM
Canning Auditorium, 3rd Floor
Prentice Women’s Hospital
250 E Superior St
Chicago, IL 60611

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