Cryorecovery/Embryo Thaw
Our cryorecovery/embryo thaw service allows the worldwide import and recovery of mouse lines from frozen embryos. Cryopreservation of mouse germplasm is rapidly becoming one of the most common methods for maintaining huge repertoires of genetically altered mice globally. Many animal vendors such as Jackson Laboratories, Taconic, Charles River, and RIKEN Bioresource Center, Experimental Animal Division have begun maintaining lines as frozen embryo and/or sperm stocks. Two NIH initiatives, the Mutant Mouse Regional Resource Centers (MMRRC) sponsored by NIH-NCRR, and the NIH Knockout Mouse Project, are charged with creating a public resource of catalogued transgenic and null mutation mouse models, respectively. The majority of the MMRRC lines and most of the knockout lines currently available from the KOMP are maintained as cryopreserved stocks. Cryopreservation of embryos is increasingly gaining popularity in academic institutions as well, as this technology becomes more mainstream.
Shipment of cryopreserved embryos is a safe, affordable way to transport mouse lines worldwide and eliminates the need to send live animals. Cryorecovery is also an acceptable method to rederive infected mouse lines.
TTML has the capability of both shipping and receiving cryopreserved embryos. Frozen embryos received from either outside the institution or stored in the TTML can be thawed and surgically transferred into recipient females (cryorecovered) at any time.
